Tip clearance losses represent a major efficiency penalty of turbine blades. This paper describes the effect of tip clearance on the aerodynamic characteristics of an unshrouded axial-flow turbine cascade under very low Reynolds number conditions. The Reynolds number based on the true chord length and exit velocity of the turbine cascade was varied from to by changing the velocity of fluid flow. The freestream turbulence intensity was varied between 0.5% and 4.1% by modifying turbulence generation sheet settings. Three-dimensional flow fields at the exit of the turbine cascade were measured both with and without tip clearance using a five-hole pressure probe. Tip leakage flow generated a large high total pressure loss region. Variations in the Reynolds number and freestream turbulence intensity changed the distributions of three-dimensional flow, but had no effect on the mass-averaged tip clearance loss of the turbine cascade.