The effect of hot streaks on deposition in a high pressure turbine vane passage was studied both experimentally and computationally. Modifications to Ohio State's Turbine Reaction Flow Rig allowed for the creation of simulated hot streaks in a four-vane annular cascade operating at temperatures up to 1093 °C. Total temperature surveys were made at the inlet plane of the vane passage, showing the variation caused by cold dilution jets. Deposition was generated by introducing sub-bituminous ash particles with a median diameter of 11.6 μm far upstream of the vane passage. Results indicate a strong correlation between surface deposits and the hot streak trajectory. A computational model was developed in Fluent to simulate both the flow and deposition. The flow solution was first obtained without particulates, and individual ash particles were subsequently introduced and tracked using a Lagrangian tracking model. The critical viscosity model was used to determine particle sticking upon impact with vane surfaces. Computational simulations confirm the migration of the hot streak and locations susceptible to enhanced deposition. Results show that the deposition model is overly sensitive to temperature and can severely overpredict deposition. Model constants can be tuned to better match experimental results but must be calibrated for each application.