In this study, the impact of single grooves at different locations on compressor stability and tip clearance flow are numerically and experimentally investigated. Initially, the numerical stall margin improvement (SMI) curve is examined using experimental data. Then, the evolution of the interface between the tip leakage flow (TLF) and the incoming main flow (MF) in the prestall and stall inception processes for two typical grooves, i.e., the worst and the optimal grooves in terms of their SMI, are compared with the smooth casing. The results show two different interface behaviors throughout the throttling process. The compressor with the worst single groove casing first experiences a long-length-scale disturbance after the interface near the blade suction side spills in front of the rotor leading-edge plane, and then goes through spikes after the whole interface spills. With the smooth casing and the optimal single groove near midchord, the interface reaches the rotor leading edge at the last stable operating point and spikes appear once the whole interface spills over the rotor leading edge. A model that illustrates the spillage patterns of the interface for the two stall precursors is thus proposed accordingly and used to explain their effectiveness in terms of the SMI. At last, the relevance of these results to the preliminary selection of groove locations for multigroove casing treatments (CTs) is verified by test data and discussed.