Multiple-injection is an effective injection strategy in order to control the advanced combustion processes in diesel engines. However, because of the multiple-injection application, cause the duration of each injection is shortened, such as the pilot injection and post-injection. The short injection duration results in a very short quasi-steady injection process so that the ramping-up and ramping-down injection processes occupied a much larger scale during the injection. As a result, this circumstance of the spray evolution not been fully understood. To investigate the diesel spray propagation with varying injection rate, visual experiments and numerical simulation analyses on diesel spray were performed. The penetrations of diesel sprays with short injection duration were obtained by reflected shadowgraphy in a combustion chamber’s constant-volume with the multi-hole injector. The diesel spray with varying injection rates was modeled by using CONVERGE CFD software and the model was calibrated and validated by the experimental data. Then diagnosed the spray characteristics including spray penetration, Sauter means diameter, as well as fuel concentration distribution, were analyzed with different injection quantities and injection rate shapes. The spray mixing analysis included that after the end-of-injection in order to consider the low-temperature combustion phenomenon. The shape of the improved injection rate in the fuel mixture considered in the case of injection ending before or after the ignition time was summarized for different conditions.