How to enlarge the output displacement is a key issue in the research field of microgrippers. It is difficult to further enlarge the output displacement for the traditional displacement transmission mechanism (DTM). In this research, a two-stage amplification cylinder-driven DTM based on the compliant mechanisms is designed to realize the displacement output expansion. The opening and closing of the clamping jaws is driven by the air cylinder to enlarge the output displacement of the microgripper. According to the analysis of statics model of the mechanism, the relationship between the output displacement of the microgripper and the driving pressure of the cylinder is established. The magnification of the microgripper is obtained using a dynamic model. Moreover, based on the finite element analysis, the mechanical structure parameters are optimized. The microgripper was fabricated by utilizing wire electro discharge machining (WEDM) technique, and then a series of experiments were carried out to obtain the relationship between the displacement and the driving pressure. It is found that the maximum output displacement measured is 1190.4 μm under the pressure of 0–0.6 MPa, corresponding to the magnification of 47.63. Compared with the results of finite element analysis and theoretical calculation, the test results have a discrepancy of 2.39% and 6.62%, respectively. The microgripper has successfully grasped a variety of micro-parts with irregular shapes, and parallel grasping can be achieved, demonstrating the potential application of this design in the field of micromanipulation.